dissemination of extended-spectrum β-lactamases and quinolone resistance genes among clinical isolates of uropathogenic escherichia coli in children
نویسندگان
چکیده
conclusions ctx-m was the most prevalent esbl genotype in uropathogenic e. coli (upec) isolated from uti. in addition, a high frequency of qnr genes among esbl-producing e. coli was identified in this study. in order to avoid treatment failures, we recommend using phenotypic and molecular methods to diagnose these enzymes and qnr genes. results the highest sensitivity was seen to imipenem (96.7%), amikacin (92.5%), nitrofurantoin (93.3%), ofloxacin (81.7%), gentamicin norfloxacin (70.8%), and ciprofloxacin (79.2%). in contrast, the highest rate of resistance was seen to co-trimoxazole (77%) and nalidixic acid (40.9%). the results showed that 6 (2.18%) and 4 (1.12%) isolates of esbl-producing e. coli were positive with respect to having qnrb and qnrs genes, respectively. no isolates was found to have qnra. materials and methods in this study, a total of 120 isolates of e. coli from urinary tract infections of the children were collected at besat hospital in hamadan, iran, from october 2010 to october 2011. the bacterial isolates were identified by standard biochemical methods. antimicrobial susceptibilities were determined by disk diffusion method, and esbls-producing was confirmed phenotypically using the double-disk synergy (dds) test. the presence and identification of esbls and qnr genes were determined by polymerase chain reaction (pcr). background urinary tract infection (uti) is one of the most common childhood bacterial infections and escherichia coli is the major pathogen. producing β-lactamase enzymes are the most common mechanism of bacterial resistance. objectives this study aimed to determine the prevalence of extended-spectrum β-lactamases (esbls) and quinolone resistance (qnr) genes in e. coli strains isolated from utis.
منابع مشابه
Prevalence of Extended-Spectrum β-Lactamases Genes in Clinical Isolates of Pseudomonas aeruginosa
ABSTRACT Background and Objectives: Pseudomonas aeruginosa is an opportunistic pathogen resistant to various antibiotics. The aim of the present study was to study resistant patterns in clinical isolates of P. aeruginosa, classify them into pandrug resistance (PDR), extensive drug resistance (XDR) and multidrug resistance (MDR)...
متن کاملPhylogenetic typing and detection of extended-spectrum β-lactamases in Escherichia coli isolates from broiler chickens in Ahvaz, Iran
This study was conducted to reveal the phylogenetic background, to detect the genes encoding TEM, SHV and CTX-M-15 extended-spectrum β-lactamases (ESBL), and to analyze their distribution in phylo-groups of 150 Escherichia coli isolates from broiler chickens in Ahvaz (Southwest of Iran). Seventy- five cloacal swabs from healthy birds (fecal isolates), and 75 heart blood samples from bi...
متن کاملStudy of Antibiotic Susceptibility Pattern and Frequency of Extended Spectrum β-lactamases Genes TEM and SHV in Urinary Tract Isolates of Escherichia coli and Klebsiella pneumoniae in Tabriz
Background & objectives: Urinary tract infection (UTI) is one of the most common types of human infections and Escherichia coli and Klebsiella pneumonia are the main causes of urinary tract infection among the gram negative bacteria. The prevalence of extended-spectrum β-lactamases (ESBLs) among these bacteria and hence resistant strains to β-lactam antibiotics have increased in recent decades...
متن کاملprevalence of quinolone resistance genes among extended-spectrum b-lactamase-producing escherichia coli in mashhad, iran
conclusions the high prevalence of quinolone resistance genes, which indicates antibiotic resistance, in the imam reza hospital of mashhad is a major concern. hence, the antibiotics prescription policy should be revised, and infection control measures should be improved. results eighty-six (43%) isolates were ciprofloxacin-resistant. the pct identified 85 (42.5%) of 200 e. coli isolates as esbl...
متن کاملPrevalence and Molecular Characterization of Plasmid-mediated Extended-Spectrum β-Lactamase Genes (balaTEM, blaCTX and blASHV) Among Urinary Escherichia coli Clinical Isolates in Mashhad, Iran
Objective(s) Extended-spectrum beta-lactamase (ESBL) producing bacteria have an important role in nosocomial infections. Due to the limited availability of information about the molecular epidemiology of ESBL producing bacteria in Mashhad, we decided to investigate about TEM, CTX and SHV ESBLs among urinary Escherichia coli isolates in Mashhad, a city in northeast Iran. Materials and Methods ...
متن کاملFrequency of E. coli clinical isolates producing blaSHV and blaTEM extended-spectrum beta-lactamases
Introduction: Production of extended-spectrum beta-lactamase enzymes (ESBLs) in E. coli creates many problems for patients. These enzymes are located on transferable elements and can hydrolyze penicillins, broad-spectrum cephalosporins, and aztreonam. This study aimed to determine the clinical isolates of E. coli producing ESBLs of blaSHV and blaTEM in the city of Zanjan. Methods: This cross...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
jundishapur journal of microbiologyجلد ۸، شماره ۷، صفحات ۰-۰
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023